Mycoplasma: Properties, Detection, and Decontamination Methods of Cell Cultures and Viral Strains
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OA Leonovich
Applied Biochemistry and Microbiology, 2024
Springer
Mycoplasma contamination of continuous cell cultures and collection viral strains remains a serious problem in the biotechnology industry and experimental research. The frequency of mycoplasma contamination of cultured cell lines and viruses is 15–35%, and in some cases up to 80%. Mycoplasmas cause various changes in cultures contaminated by them, including cell death. They have immunomodulatory properties and affect the yield of certain viruses propagated in cell cultures. Mycoplasmas do not have a cell wall, are able to pass …
