Enhancement or Activation of Agricultural Antibiotic Production in Streptomyces by an Identified Strong Promoter P30S
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B Huang, Y Shi, H Qi, J Wang, Y Zhang…
Journal of Agricultural …, 2025
ACS Publications
We first screened for strong constitutive promoters by analyzing transcriptomics data under various fermentation conditions, focusing on genes with sustained high expression levels. Then, we validated the expression strength of 21 candidate promoters using β-glucuronidase enzymatic activity and qRT-PCR assays and observed that promoter P30S demonstrated consistently strong expression and efficacy in Streptomyces rimosus M527. The higher expression level and utility of P30S were further confirmed in three different host …
