期刊论文Collagen and picrosirius red staining: a polarized light assessment of fibrillar hue and spatial distribution复制

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L Rich, P Whittaker
Braz. j. morphol. sci, 2005
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Collagen plays a vital role in maintaining structural integrity and in determining tissue function. Therefore, methods to detect, quantify, and analyze collagen are valuable. Nevertheless, stains historically employed to detect collagen have disadvantages, principally a poor specificity for thin fibers. Conversely, picrosirius red, which has the capability to detect thin fibers, although frequently used, is seldom exploited to the fullest extent. Our goal was, using picrosirius red staining, circularly polarized light, and image …