FX-Cell: a method for single-cell RNA sequencing on difficult-to-digest and cryopreserved plant samples
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X Ming, MC Wan, ZD Zhang, HC Xue, YQ Wu, ZG Xu…
Nature …, 2025
nature.com
Single-cell RNA sequencing in plants requires the isolation of high-quality protoplasts—cells devoid of cell walls. However, many plant tissues and organs are resistant to enzymatic digestion, posing a significant barrier to advancing single-cell multi-omics in plant research. Furthermore, for field-grown crops, the lack of immediate laboratory facilities presents another major challenge for timely protoplast preparation. Here, to address these limitations, we developed FX-Cell and its derivatives, FXcryo-Cell and cryoFX-Cell, to enable single-cell …
