mRNA display pipeline for protein biosensor construction
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Z Cui, CE Ayva, YJ Liew, Z Guo, R Mutschler…
ACS …, 2024
ACS Publications
Despite the significant potential of protein biosensors, their construction remains a trial-and-error process. The most obvious approach for addressing this is to utilize modular biosensor architectures where specificity-conferring modalities can be readily generated to recognize new targets. Toward this goal, we established a workflow that uses mRNA display-based selection of hyper-stable monobody domains for the target of choice or ribosome display to select equally stable DARPins. These binders were integrated into a two-component …
