Multiplex genome editing eliminates lactate production without impacting growth rate in mammalian cells
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H Hefzi, I Martínez-Monge, I Marin de Mas…
Nature …, 2025
nature.com
The Warburg effect, which describes the fermentation of glucose to lactate even in the presence of oxygen, is ubiquitous in proliferative mammalian cells, including cancer cells, but poses challenges for biopharmaceutical production as lactate accumulation inhibits cell growth and protein production. Previous efforts to eliminate lactate production in cells for bioprocessing have failed as lactate dehydrogenase is essential for cell growth. Here, we effectively eliminate lactate production in Chinese hamster ovary and in the human …
