Construction and identification of a sizeable naive human Fab phage display antibody library
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YK Zhao, XY Wei, FL Meng, WT Liu…
Zhonghua liu xing …, 2025
pubmed.ncbi.nlm.nih.gov
Objective: To construct a sizeable naive human Fab phage display antibody library to screen high-affinity specific antibodies in vitro. Methods: Total RNA was extracted from peripheral blood mononuclear cells (PBMCs) of 126 healthy individuals, subsequently reverse-transcribed into cDNA, and used as a template. PCR amplification was performed to obtain the VH from IgG, IgM and light chain κ, λ, separately, with the initial PCR products serving as templates for a second round of PCR. Overlap extension PCR was employed to generate …
