Generation of a phage display chicken single-chain variable fragment library
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H Yang, J Chae, H Kim, J Noh…
Cold Spring Harbor …, 2025
cshprotocols.cshlp.org
Phage-displayed antibody fragment libraries can be constructed using essentially any species that is easily immunized, as long as the immunoglobulin variable region gene sequences are known. This protocol describes the procedures for the generation of a phage-displayed chicken single-chain variable fragment (scFv) library after immunization with a target antigen. Briefly, the rearranged heavy chain variable region (VH) genes and the λ light chain variable region (V λ) genes are amplified separately and are linked through two …
