Development of a monoclonal antibody-based sandwich ELISA for detection and quantification of the major cat allergen Fel d 1
DOI:
文献链接: https://doi.org/10.1016/j.ijbiomac.2025.147129
其他信息:
Z Bi, L Cai, Z Zhang, Q Du, S Zhu, M Zhang…
International Journal of …, 2025
Elsevier
Felis domesticus allergen 1 (Fel d 1), the primary allergen produced by cats, is a key trigger of various allergic symptoms in humans, such as rhinitis, conjunctivitis, and asthma. In this study, a recombinant Fel d 1 protein composed of chain 2 and chain 1 was successfully produced in E. coli, based on a previously reported fusion construct that closely approximates the native allergen. Two antibodies, 2A7 and 6B1, were selected for further study due to their high specificity and complementary epitope recognition. A dual-antibody …
