Analysis of Oligonucleotide Stability and Fragmentation: Impact of 2′‐Modifications, Base Composition, and Charge‐States
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C Gawlig, M Kaumann, G Hanci…
… in Mass Spectrometry, 2026
Wiley Online Library
Rationale Mass spectrometry‐based sequencing of therapeutic oligonucleotides requires controlled fragmentation, but the effects of nucleotide composition, charge state, and chemical modification on collision‐induced dissociation are not fully understood. This study systematically evaluates how these parameters influence precursor survival, base loss, and backbone fragmentation to improve and understand analytical characterization and fragmentation behavior of modified oligonucleotides. Methods Homopolymeric 15‐mer DNA …
