Detection of Cellular Senescence on Murine Muscle Tissue Sections by Senescence-Associated β-Galactosidase Staining
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W Zhang, TH Cheung
2025
Springer
Staining for the presence of senescence-associated beta-galactosidase (SA-β-gal) serves as a crucial indicator for cellular senescence. This staining assay is based on the elevated activity of β-galactosidase in senescent cells, which cleaves the X-Gal substrate to produce an insoluble blue product in acidic conditions. This enables X-Gal to be visualized using microscopy. In this study, we describe the identification of SA-β-gal+ cells within murine muscle tissues.
