Mechanistic insights into RNA cleavage by human Argonaute2–siRNA complex
1. 当前求助状态已完结, 请及时下载应助文件
2. 系统将在 2025-07-31 15:41:40 删除文件
注: 所有应助的资源仅供学习交流使用, 不得违反相关法律法规
DOI:
文献链接:
其他信息:
Z Li, Q Xu, Y Zhang, J Zhong, T Zhang, J Xue, S Liu…
Cell Research, 2025
nature.com
In animals, AGO-clade Argonaute proteins utilize small interfering RNAs (siRNAs) as guides to recognize target with complete complementarity, resulting in target RNA cleavage that is a critical step for target silencing. These proteins feature a constricted nucleic acid-binding channel that limits base pairing between the guide and target beyond the seed region. How the AGO–siRNA complexes overcome this structural limitation and achieve efficient target cleavage remains unclear. We performed cryo-electron microscopy of human AGO–siRNA …
